https://molmed.biomedcentral.com/articles/10.1186/s10020-020-00157-3
https://www.ncbi.nlm.nih.gov/pubmed/32450789?dopt=Abstract
TITLE:
Down-regulated miR-495 can target programmed cell death 10 in ankylosing spondylitis.
DESCRIPTION:
Related Articles
Down-regulated miR-495 can target programmed cell death 10 in ankylosing spondylitis.
Mol Med. 2020 May 25;26(1):50
Authors: Ni WJ, Leng XM
Abstract
BACKGROUND: MicroRNAs (miRNAs) play crucial roles in regulating eukaryotic gene expression. Recent studies indicated that aberrantly expressed miRNAs are involved in the pathogenesis of ankylosing spondylitis (AS). Indeed, hsa-miR-495-3p (miR-495) has been reported as an anti-oncogene in different cancers. However, the role of miR-495 in AS is still unknown.
METHODS: In this study, quantitative real-time polymerase chain reaction (PCR) was used to detect the expression of miR-495 in the peripheral blood mononuclear cells (PBMCs), whole blood, and serum of patients with AS. Bisulfite-specific PCR sequencing and methylated DNA immunoprecipitation were used to detect the methylation in the promoter region of miR-495. To determine the influence of miR-495 expression on the target gene, programmed cell death 10 (PDCD10), dual luciferase reporter assays together with an adenoviral vector containing the miR-495 locus were used. Receiver operating characteristic (ROC) curves were used to evaluate the efficacy of miR-495 as a diagnostic biomarker of AS. Gene Ontology, Kyoto Encyclopedia of Genes and Genomes pathway analysis, and western blotting were used to explore the potential role of miR-495 in AS pathogenesis and the mechanism by which it facilitates AS pathogenesis.
RESULTS: miR-495 is down-regulated and the promoter region of miR-495 is highly methylated in AS. The expression of miR-495 is negatively associated with PDCD10 expression in both patients with AS and healthy controls. Further experiments showed that PDCD10 can be targeted by miR-495. The ROC curves of miR-495 suggested that it is a very specific and sensitive biomarker for AS diagnosis. Bioinformatics analysis and signal pathway studies indicated that miR-495 can down-regulate β-catenin and transforming growth factor-β1.
CONCLUSIONS: Our studies indicated that down-regulation of miR-495 can be used as a potential molecular marker for the diagnosis and treatment of AS, thus providing new insights into the role of miRNAs in AS pathology.
PMID: 32450789 [PubMed – in process]
PMID:
PubMed:32450789
DATE FOUND:
05/27/20 07:21AM
LINK / URL:
https://www.ncbi.nlm.nih.gov/pubmed/32450789?dopt=Abstract